Class-Specific Protein Assay Kits
Bioassay Systems QuantiChrom™ G6PDH Inhibitor Screening Kit. HTS for inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.
For evaluation and high-throughput screen (HTS) of glucose-6-phosphate dehydrogenase modulators. Key Features: Safe. Non-radioactive assay. High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in 30 minutes and no 37°C heater is needed. Method: OD565nm. Samples: Glucose-6-phosphate dehydrogenase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.
Bioassay Systems QuantiFluo™ DNA Assay Kit. For quantitative determination of DNA. For quantitative determination of DNA.
For quantitative determination of DNA. Key Features: Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 - 2,000 ng/mL) calf thymus DNA in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day. Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay. Method: FL350/450nm. Samples: Plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 100 ng/mL.
Bioassay Systems EnzyLight™ Cytotoxicity Assay Kit. For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxicity and high-throughput screen for anticancer agents.
For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 50 cells can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells. Species: All. Procedure: Assay takes 2 min. Kit size: 100 tests. Detection limit: 50 cells.
Bioassay Systems EnzyChrom™ Invertase Assay Kit. For quantitative determination of invertase/sucrase activity.
For quantitative determination of invertase/sucrase enzyme activity. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.007 U/L invertase activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological, environment (soil) etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.007 U/L.
Bioassay Systems EnzyChrom™ Maltose Assay Kit.Quantitative determination of maltose in various biological samples such as serum, urine, food and beverages, etc.
For quantitative determination of maltose and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 10 µL sample. Linear detection range 2 to 500 µM maltose for colorimetric assays and 1 to 50 µM for flourimetric assays. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, urine, food and beverages, etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 2 µM.
Bioassay Systems EnzyChrom™ Xanthine Oxidase Assay Kit. For quantitative determination of xanthine oxidase enzyme activity.
For quantitative determination of xanthine oxidase enzyme activity. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 20 minute incubation: 0.03 to 25 U/L xanthine oxidase for colorimetric assays and 0.01 to 2.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Method: OD570nm, FL530/585nm; Samples: Cell lysate, serum, and other biological samples; Size: 100 tests.
Bioassay Systems Parallel Artificial Membrane Permeability Assay (PAMPA) Kit. For assess gastrointestinal (GI) membrane permeability of test compounds.
For quantitative determination of gastrointestinal (GI) membrane permeability of test compounds. Key Features: Convenient. Includes all necessary equipment to run a PAMPA plate. Simple and low-cost. Procedure is easy to follow and more affordable than cell-based permeability assays. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: PAMPA. Samples: Chemical compounds. Species: All. Procedure: Assay takes 20 hr, hands-on time 1 hr. Kit size: 96 tests.
Bioassay Systems QuantiFluo™ Diamine Oxidase Assay Kit. For quantitative determination of diamine oxidase in biological samples (e.g. serum, plasma etc).
For quantitative determination of diamine oxidase and evaluation of drug modulators. Key Features: Fast and sensitive. Use of 10 µL sample. Linear detection range 0.5 to 6 U/L for 30 min reaction at 25°C. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm. Samples: Serum, Plasma. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.
Bioassay Systems QuantiChrom™ Biotin Assay Kit. Quantitative determination of biotin in food, cosmetic, and biotinylated proteins.
For quantitative determination of biotin in food, cosmetic, and biotinylated proteins. Key Features: Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well). Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD500nm. Samples: Food, cosmetics, supplements, and biotinylated proteins or antibodies. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 8 µM.
Bioassay Systems EnzyFluo™ Tryptophan Assay Kit. Direct determination of tryptophan in serum.
For direct determination of tryptophan in serum. Key Features: Fast and sensitive. Use of 10 µL sample. Linear detection range from 10 µM to 400 µM tryptophan in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 30 minutes. Room temperature assay. No 37 °C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm; Samples: Serum; Size: 100 wells.
Bioassay Systems QuantiChrom™ Arginase Inhibitor Screening Kit. HTS for inhibitor screening and evaluation of arginase inhibitors.
For evaluation and high-throughput screen (HTS) of arginase modulators. Key Features: Safe. Non-radioactive assay. High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in less than 2 hours and no 37°C heater is needed. Method: OD430nm. Samples: Arginase. Species: All. Procedure: Assay takes 1 hour 45 min. Kit size: 100 tests.
Bioassay Systems QuantiChrom™ Β-glucosidase Assay Kit. For quantitative determination of β-glucosidase activity and evaluation of drug effects on its metabolism.
For quantitative determination of β-glucosidase activity and evaluation of drug effects on its metabolism. Key Features: High sensitivity and wide linear range. Use 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 2 U/L.
Bioassay Systems QuantiFluo™ Acid Phosphatase Assay Kit. Acid Phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)
For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.008 to 10 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.008 U/L.
Eurofins Discoverx PathHunter eXpress GPR142 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0413E2ACP1M)
The PathHunter GPR142 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR142 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR142 and EA-tagged β-Arrestin-2. Activation of GPR142 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences formate Dehydrogenase from
formateNAD oxidoreductaseformate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
